Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts | Zendy

T.S. Tenforde | Zendy; Jack Risius | Zendy; Aviva G. Beckmann | Zendy; Cornelius A. Tobias | Zendy; E G Gurney | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts

Author(s) -

T.S. Tenforde,

Jack Risius,

Aviva G. Beckmann,

Cornelius A. Tobias,

E G Gurney

Publication year - 1975

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/4148367

Subject(s) - dissociation (chemistry) , immunosuppression , in vivo , microbiology and biotechnology , virus , fibroblast , chemistry , simian , cell , cell culture , in vitro , biology , virology , cancer research , biochemistry , immunology , genetics

Mouse fibroblasts transformed by Simian Virus 40 (SV40) were examined for tumor forming ability in syngeneic BALB/c mice following dissociation from tissue culture dishes by two procedures. A significantly greater in vivo proliferative capacity was observed for cells dissociated by the tryspin-EDTA procedure, with the injected cell dose for tumor production in 50 percent of recipient mice (the TPD$sub 50$) being 16-fold lower than the TPD$sub 50$ for cells dissociated by the EDTA procedure. Host immunosuppression with 300 rad whole-body $gamma$ irradiation led to a significant 7-fold decrease in the TPD$sub 50$ for cells dissociated by the EDTA procedure, while no significant decrease in TPD$sub 50$ was observed for cells dissociated by the tryspin-EDTA procedure. (auth

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research