Coupling sequencing by hybridization (SBH) with gel sequencing for an inexpensive analysis of genes and genomes | Zendy

Snezana Drmanac | Zendy; Ivan Labat | Zendy; Brian Hauser | Zendy; Rade Drmanac | Zendy

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Coupling sequencing by hybridization (SBH) with gel sequencing for an inexpensive analysis of genes and genomes

Author(s) -

Snezana Drmanac,

Ivan Labat,

Brian Hauser,

Rade Drmanac

Publication year - 1996

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/405751

Subject(s) - sequencing by hybridization , dna sequencing , dna sequencer , biology , dna , genome , genetics , computational biology , dna–dna hybridization , gene

The speed and cost of DNA sequencing are bottlenecks in the analysis of genes end genomes. Sequencing by hybridization (SBH) is a versatile method with several applications which can accelerated DNA screening, mapping and sequencing. Requirements, achievements and problems in the development of the SBH format 1 (DNA samples arrayed) are presented and schemes for its synergetic coupling with gel sequencing techniques are discussed. It appears that by one hybridization machine with 24 boxes and four ABI gel sequencers 100- 300 Mb of DNA sequence can be determined per year. Various genetic studies based on computer assisted analysis of large collections of partial or complete DNA sequences (`sequenetics`) may be achieved in this century

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