Open AccessAdvanced biomolecular materials based on membrane-protein/polymer complexation
Author(s) -
Gregory S. Smith,
Andrew P. Nowak,
Cyrus R. Safinya
Publication year - 1998
Language(s) - English
Resource type - Reports
DOI - 10.2172/296874
Subject(s) - neutron reflectometry , membrane , national laboratory , polymer , materials science , reflectometry , thin film , neutron scattering , sample (material) , lipid bilayer , nanotechnology , neutron , chemistry , computer science , small angle neutron scattering , chromatography , physics , engineering physics , composite material , nuclear physics , biochemistry , computer vision , time domain
This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The goal of this project was to apply neutron reflectometry and atomic force microscopy to the study of lipid membranes containing proteins. Standard sample preparation techniques were used to produce thin films of these materials appropriate for these techniques. However, these films were not stable, and a new sample preparation technique was required. Toward this goal, the authors have developed a new capability to produce large, freely suspended films of lipid multi-bilayers appropriate for these studies. This system includes a controlled temperature/humidity oven in which the films 5-cm x 5-cm are remotely drawn. The first neutron scattering experiments were then performed using this oven