Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1, 1995--December 31, 1995 | Zendy

N.C. Mishra | Zendy

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Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1, 1995--December 31, 1995

Author(s) -

N.C. Mishra

Publication year - 1995

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/245631

Subject(s) - neurospora , tyrosinase , bioconversion , enzyme , oligonucleotide , biochemistry , coal , chemistry , enzyme assay , solubilization , sequence (biology) , oxidase test , amino acid , microbiology and biotechnology , gene , biology , chromatography , organic chemistry , fermentation , mutant , neurospora crassa

The ability of Neurospora to solubilize and bioconvert coal was investigated. The coal solubilizing activity (CSA) was fractionated to isolate the enzyme responsible for this activity. The enzyme was purified in order to obtain the amino acid sequence. From that sequence potential oligonucleotide probes were synthesized and used to screen genomic library of Neurospora. The gene so identified was isolated. CSA appears to be an phenol oxidase or is tyrosinase

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