Open AccessMapping and ordered cloning of the human X chromosome. Final progress report, March 1991--February 1995
Author(s) -
C. Thomas Caskey
Publication year - 1995
Language(s) - English
Resource type - Reports
DOI - 10.2172/211497
Subject(s) - cosmid , cloning (programming) , genetics , complementary dna , biology , computational biology , chromosome , dna , microbiology and biotechnology , computer science , gene , programming language
A reciprocal probing method is described which uses pooled cDNA probes to order chromosome specific libraries in order to identify cosmids containing sequences capable to hybridizing to the pool. In this pilot study, placental DNA clones were used to identify cosmids from both chromosomes X and 17. Sixty unique cDNA`s were identified of which 22 were novel