Open AccessModeling single molecule detection probabilities in microdroplets. Final report
Author(s) -
Steven C. Hill
Publication year - 1996
Publication title -
osti oai (u.s. department of energy office of scientific and technical information)
Language(s) - English
Resource type - Reports
DOI - 10.2172/206995
Subject(s) - laser linewidth , numerical aperture , molecule , optics , orientation (vector space) , position (finance) , lens (geology) , fluorescence , materials science , physics , geometry , mathematics , laser , wavelength , finance , quantum mechanics , economics
Numerical tools for modeling the fluorescence collected from a single molecule within a microsphere as a function of it`s position and orientation, the size of the droplet, the numerical aperture of the lens, the detection geometry, type of illumination, and the linewidth of the emitting molecule, are described
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