A novel biomarker for beryllium sensitization in humans. 1997 annual progress report | Zendy

R.J. Albertini | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A novel biomarker for beryllium sensitization in humans. 1997 annual progress report

Author(s) -

R.J. Albertini

Publication year - 1997

Publication title -

osti oai (u.s. department of energy office of scientific and technical information)

Language(s) - English

Resource type - Reports

DOI - 10.2172/13585

Subject(s) - t cell receptor , beryllium , mutant , in vivo , sensitization , in vitro , hypoxanthine guanine phosphoribosyltransferase , t cell , mutation , microbiology and biotechnology , biology , immunology , gene , cancer research , chemistry , genetics , immune system , organic chemistry

'Overall, this project is designed to identify the beryllium reactive T-cell clones that are proliferating in vivo in individuals sensitized to beryllium. The basic method for identifying such clones is the hprt T-cell mutation assay. The rationale is that in vivo proliferating T-lymphocytes are more likely to undergo hprt mutations and/or be included in hprt mutant fractions of T-cells isolated from peripheral blood. T-lymphocytes isolated as hprt mutants from beryllium sensitized individuals are propagated in vitro and characterized for T-cell receptor (TCR)/3 gene usage patterns and antigen reactivity. Results will be compared with similar characteristics determined for T-cell clones from the same individuals that were developed in vitro from peripheral blood lymphocytes by beryllium stimulation. This research project has several specific aims. Progress for the year 10/1/96 to 9/30/97 is given.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research