PHOTOBIOLOGICAL HYDROGEN RESEARCH | Zendy

George P. Philippidis | Zendy; Vekalet Tek | Zendy

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PHOTOBIOLOGICAL HYDROGEN RESEARCH

Author(s) -

George P. Philippidis,

Vekalet Tek

Publication year - 2009

Language(s) - English

Resource type - Reports

DOI - 10.2172/1130089

Subject(s) - hydrogenase , gene , cloning (programming) , protein subunit , escherichia coli , strain (injury) , biohydrogen , chemistry , computational biology , enzyme , hydrogen production , biology , biochemistry , catalysis , computer science , anatomy , programming language

The project objectives are to develop bio-hydrogen production by: Cloning the structural and subunit genes (cooKMUX and cooLH resp.) of the O{sub 2}- tolerant NiFe-hydrogenase from the photosynthetic bacterium Rubrivivax gelatinosus CBS strain in collaboration with NREL. Cloning the active site maturation genes (hypA-F) of the CBS hydrogenase in collaboration with NREL. Transforming the structural and subunits genes, along with the maturation genes, into E. coli and determining the minimum number of genes required for expression of a functional hydrogenase. Upon expression of a functional hydrogenase, purifying and characterizing the recombinant hydrogenase from E. coli and performing bioreactor studies to optimize hydrogen production by E. coli.

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