Open AccessIntegrated Genome-Based Studies of Shewanella Ecophysiology
Author(s) -
Alfred M. Spormann
Publication year - 2011
Language(s) - English
Resource type - Reports
DOI - 10.2172/1077855
Subject(s) - shewanella oneidensis , mutant , biofilm , biology , shewanella , computational biology , genetics , biochemistry , microbiology and biotechnology , gene , bacteria
We have constructed in-frame deletions of 7 of the 10 PAS-GGDEF-EAL proteins in Shewanella oneidensis MR-1. We are currently in the process of characterizing the deletion mutants under a wide range of growth conditions. In addition to characterizing growth, we will also examine the biofilm formation of the deletion mutants. In addition to the genetic analyses of the mutants, we are also interested in comparing the activities of the various PAS-GGDEF-EAL proteins. Proteins containing PAS, GGDEF and EAL amino acid sequence motifs may play an important role in regulating c-di-GMP signaling in response to environmental conditions. A genetic and biochemical analysis into the roles of these proteins is underway. PDE activity was observed for several PAS-GGDEF-EAL proteins. One of these proteins, SO0427, also demonstrates possible DGC activity in vitro. Currently, we are studying the growth, motility and biofilm formation characteristics of deletion mutants, as well as the activity of the purified proteins