Open AccessRapid Automated Sample Preparation for Biological Assays
Author(s) -
Maxim Shusteff
Publication year - 2011
Language(s) - English
Resource type - Reports
DOI - 10.2172/1021552
Subject(s) - lysis , extraction (chemistry) , chip , dna extraction , sample (material) , task (project management) , computer science , filter (signal processing) , lab on a chip , sample preparation , process engineering , chromatography , computer hardware , embedded system , materials science , nanotechnology , chemistry , engineering , microfluidics , biology , microbiology and biotechnology , polymerase chain reaction , systems engineering , computer vision , telecommunications , biochemistry , gene
Our technology utilizes acoustic, thermal, and electric fields to separate out contaminants such as debris or pollen from environmental samples, lyse open cells, and extract the DNA from the lysate. The objective of the project is to optimize the system described for a forensic sample, and demonstrate its performance for integration with downstream assay platforms (e.g. MIT-LL's ANDE). We intend to increase the quantity of DNA recovered from the sample beyond the current {approx}80% achieved using solid phase extraction methods. Task 1: Develop and test an acoustic filter for cell extraction. Task 2: Develop and test lysis chip. Task 3: Develop and test DNA extraction chip. All chips have been fabricated based on the designs laid out in last month's report