EFFECT OF COMBINED BETWEEN MICROBUBBLE AND ULTRASONIC OF C-PHYCOCYANIN EXTRACTION FROM S. PLATENSIS

The aims of this work were to approve the performance of the combination between microbubble and ultrasonic with controlled temperature on CPC extraction from S. platensis. The influence of microbubble combined with ultrasonic and controlled temperature (MBUT) was investigated under various ultrasonic frequency (28, 45, 100 kHz), various of microbubble size (30, 40, 50 μm) and various of controlled temperature (308.15, 313.15, 318.15 K). This study suggested that MBUT employed reduce the extraction time, and improved extraction efficiency namely diffusion coefficient and enhancement factor. The diffusion coefficient obtained from experimental data was in the range of 1.959×10-11 to 2.415×10-11 m2.s-1. The modified correlation was established based on theoretical diffusion in liquid (Stoke & Einstein equation). The correlation of solution viscosity as functions of microbubble size, ultrasonic frequency, and temperature was also established as an empirical model for calculating the viscosity using in the modified model. A good agreement between experimental data and the modified correlation predicted was achieved lower than 15% as the reported of %Error. Thus, these combination techniques were found to be effective for the extraction of CPC from wet S. platensis and this technique can be employed for other bioactive compounds.