Isolation, screening, and crude oil degradation characteristics of hydrocarbons-degrading bacteria for treatment of oily wastewater | Zendy

Xiumei Tian | Zendy; Xiaoli Wang | Zendy; Shitao Peng | Zendy; Zhi Wang | Zendy; Ran Zhou | Zendy; He Tian | Zendy

Open Access

Isolation, screening, and crude oil degradation characteristics of hydrocarbons-degrading bacteria for treatment of oily wastewater

Author(s) -

Xiumei Tian,

Xiaoli Wang,

Shitao Peng,

Zhi Wang,

Ran Zhou,

He Tian

Publication year - 2018

Publication title -

water science and technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.406

H-Index - 137

eISSN - 1996-9732

pISSN - 0273-1223

DOI - 10.2166/wst.2019.025

Subject(s) - pristane , biodegradation , phytane , bioremediation , petroleum , chemistry , environmental chemistry , hydrocarbon , microbial biodegradation , degradation (telecommunications) , wastewater , bacteria , pseudomonas , gas chromatography , contamination , pulp and paper industry , microorganism , environmental science , chromatography , environmental engineering , organic chemistry , biology , ecology , telecommunications , genetics , computer science , engineering

The aim of this study was to isolate hydrocarbons-degrading bacteria for treatment of oily wastewater from long-standing petroleum-polluted sediments in Bohai Bay, China. Six hydrocarbons-degrading bacteria were screened and identified as Pseudomonas sp. and Bacillus sp. A new approach using a combination of various bacterial species in petroleum biodegradation was proposed and evaluated for its degradation characteristics. Gas chromatography-flame ionization detection (GC-FID) analysis showed that mixed bacterial agents (YJ01) degraded 80.64% of crude oil and 76.30% of crude oil alkanes, exhibiting good biodegradation effect. Besides, after 14 days of culture, the biodegradation assessment markers, pristane and phytane, showed significant degradation rates of 46.75% and 78.23%, respectively. Kinetic analysis indicated that the degradation trends followed a single first-order kinetics model and the degradation half-life ( 1/2 ) of 15 g/L crude oil was significantly shorter (5.48 days). These results indicated that YJ01 could degrade a wider range of hydrocarbons as well as some recalcitrant hydrocarbon components, and can be applied for bioremediation and treatment of oil-contaminated environment.

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