Prevalence and characterization of Escherichia coli in the Kelantan River and its adjacent coastal waters

The presence of Escherichia coli in river and sea water may cause different levels of infections and constitutes a risk to public health. In this study, water samples were collected from 15 sites along the Kelantan River, estuaries and its adjacent coastal waters to investigate the prevalence and diversity of E. coli. A membrane filtration technique was used to enumerate E. coli and phylogenetic grouping was performed using triplex polymerase chain reaction. E. coli abundance ranged from 3.1 × 10 to 1.6 × 10 colony forming units 100 mL , and total suspended solids correlated significantly with E. coli abundance (r1⁄4 0.165, p< 0.001) and rainfall (r1⁄4 0.342, p< 0.001). Phylogenetic group B1 and A (59.4%) were the most prevalent, whereas groups B2 and D were least abundant. The higher abundance of phylogenetic group D at upstream sites of the Kelantan River suggested fecal contamination mainly of animal origin. Canonical-correlation analysis showed phylogenetic group B2, and phylogenetic groups A and D were greater in waters with higher inorganic nutrients (e.g. NH4, NO2 and NO3), whereas phylogenetic group B1 appeared to have better salinity tolerance between phylogenetic groups. Chui Wei Bong (corresponding author) Siong Kiat Chai Choon Weng Lee Laboratory of Microbial Ecology, Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: cwbong@um.edu.my Chui Wei Bong Siong Kiat Chai Lay Ching Chai Choon Weng Lee Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia Siong Kiat Chai Institude of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia Ai Jun Wang Laboratory for Coast and Ocean Geology, Third Institute of Oceanography, Ministry of Natural Resources of People’s Republic of China, Xiamen 361005, China