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In this study, an upflow anaerobic sludge blanket (UASB) bioreactor was sequentially subjected to high-strength synthetic, low-strength synthetic and domestic wastewaters. From COD removal data, supported by volumetric loading rate, hydraulic retention time, pH and qualitative biogas production data, it was observed that the biomass in the bioreactor took about twice the time required to acclimatize to a change in substrate characteristics or composition compared to a much more drastic quantitative change, i.e., more than 95% difference, in substrate concentration. As the initial experiment coincided with the bioreactor start-up, it could also be concluded that the feeding regime did not shorten the overall start-up time of a UASB bioreactor meant to treat domestic wastewater, but its eventual success was probably more assured.

Biomass Acclimatization to Sequentially Varying Substrates in an Upflow Anaerobic Sludge Blanket (UASB) Bioreactor