Development of a qPCR assay for the detection of naturalized wastewater E. coli strains | Zendy

Shuai Zhi | Zendy; Graham Banting | Zendy; Norman F. Neumann | Zendy

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Development of a qPCR assay for the detection of naturalized wastewater E. coli strains

Author(s) -

Shuai Zhi,

Graham Banting,

Norman F. Neumann

Publication year - 2022

Publication title -

journal of water and health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.482

H-Index - 59

eISSN - 1996-7829

pISSN - 1477-8920

DOI - 10.2166/wh.2022.014

Subject(s) - wastewater , sewage , escherichia coli , biology , microbiology and biotechnology , sewage treatment , polymerase chain reaction , effluent , plasmid , feces , contamination , human feces , fecal coliform , dna , environmental engineering , water quality , environmental science , ecology , genetics , gene

We recently demonstrated the presence of naturalized populations of Escherichia coli in municipal sewage. We wanted to develop a quantitative polymerase chain reaction (qPCR) assay targeting the uspC-IS30-flhDC marker of naturalized wastewater E. coli and assess the prevalence of these naturalized strains in wastewater. The limit of detection for the qPCR assay was 3.0 × 10−8 ng of plasmid DNA template with 100% specificity. This strain was detected throughout the wastewater treatment process, including treated effluents. We evaluated the potential of this marker for detecting municipal sewage/wastewater contamination in water by comparing it to other human and animal markers of fecal pollution. Strong correlations were observed between the uspC-IS30-flhDC marker and the human fecal markers Bacteroides HF183 and HumM2, but not animal fecal markers, in surface and stormwater samples. The uspC-IS30-flhDC marker appears to be a potential E. coli-based marker for human wastewater contamination.

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