Evaluation of a most probable number method for the enumeration of Legionella pneumophila from North American potable and nonpotable water samples | Zendy

Ray Petrisek | Zendy; Jonathon Hall | Zendy

Open Access

Evaluation of a most probable number method for the enumeration of Legionella pneumophila from North American potable and nonpotable water samples

Author(s) -

Ray Petrisek,

Jonathon Hall

Publication year - 2017

Publication title -

journal of water and health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.482

H-Index - 59

eISSN - 1996-7829

pISSN - 1477-8920

DOI - 10.2166/wh.2017.118

Subject(s) - enumeration , legionella pneumophila , potable water , legionella , wastewater , microbiology and biotechnology , environmental science , mathematics , environmental engineering , biology , bacteria , combinatorics , genetics

This study compares the performance of a novel most probable number (MPN) method (Legiolert™/Quanti-Tray ® ) with Standard Methods for the Examination of Water and Wastewater 9260 J for the enumeration of Legionella pneumophila from potable and nonpotable waters. Data from the study showed that Legiolert exhibited higher sensitivity for the detection of L. pneumophila for potable water and equivalent sensitivity for nonpotable water. The Legiolert medium had a high specificity with no false positive signals reported for either water type. The new method represents a significant improvement in usability and accuracy in the enumeration of L. pneumophila.

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