Cryptosporidium genotypes and subtypes distribution in river water in Iran | Zendy

Mohammad Reza Mahmoudi | Zendy; Ehsan Nazemalhosseini Mojarad | Zendy; Bahram Kazemi | Zendy; Ali Haghighi | Zendy; Asad Mirzaei | Zendy; Anita Mohammadiha | Zendy; S. Jahantab | Zendy; Lihua Xiao | Zendy; Panagiotis Karanis | Zendy

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Cryptosporidium genotypes and subtypes distribution in river water in Iran

Author(s) -

Mohammad Reza Mahmoudi,

Ehsan Nazemalhosseini Mojarad,

Bahram Kazemi,

Ali Haghighi,

Asad Mirzaei,

Anita Mohammadiha,

S. Jahantab,

Lihua Xiao,

Panagiotis Karanis

Publication year - 2014

Publication title -

journal of water and health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.482

H-Index - 59

eISSN - 1996-7829

pISSN - 1477-8920

DOI - 10.2166/wh.2014.234

Subject(s) - cryptosporidium , genotype , biology , 18s ribosomal rna , cryptosporidium parvum , veterinary medicine , polymerase chain reaction , surface water , immunomagnetic separation , water quality , microbiology and biotechnology , ribosomal rna , feces , gene , ecology , environmental science , environmental engineering , genetics , medicine

Little is known about the diversity and public health significance of Cryptosporidium species in river waters in Iran. In the present study, we determined the genotype and subtype distribution of Cryptosporidium spp. in river water samples in Iran. A total of 49 surface water samples were collected from rivers and surface water in Guilan and Tehran provinces during 2009-2010. Water samples were filtrated through a 1.2-μm pore size membrane filter or by Filta-Max filter followed by immunomagnetic separation or sucrose purification methods. Genotype and subtype of Cryptosporidium were identified by sequence analysis of the 18S rRNA and 60 kDa glycoprotein (gp60) genes, respectively. A total of 24 (48.97%) water samples were positive for Cryptosporidium species by the 18sRNA-based polymerase chain reaction (PCR)-sequencing technique. DNA sequencing revealed the presence of five species of Cryptosporidium (C. parvum, C. hominis, C. muris, C. andersoni, and C. canis) in the water samples of the study area and, to our knowledge, the first report of C. muris in Iran. The results of GP60 gene analysis showed that all C. parvum and C. hominis isolates belonged to the IId and Id subtype families, respectively. The investigated river water supplies were heavily contaminated by pathogenic species of Cryptosporidium from humans and livestock. There is potential risk of waterborne cryptosporidiosis in humans and animals.

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