Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes | Zendy

Hodon Ryu | Zendy; Brooke K. Mayer | Zendy; Morteza Abbaszadegan | Zendy

Open Access

Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes

Author(s) -

Hodon Ryu,

Brooke K. Mayer,

Morteza Abbaszadegan

Publication year - 2009

Publication title -

journal of water and health

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.482

H-Index - 59

eISSN - 1996-7829

pISSN - 1477-8920

DOI - 10.2166/wh.2009.208

Subject(s) - cryptosporidium , enteric virus , microbiology and biotechnology , biology , echovirus , cryptosporidium parvum , real time polymerase chain reaction , coxsackievirus , virology , enterovirus , virus , feces , biochemistry , gene

This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

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