ASSESSING GENETIC DIVERSITY OF SOME POTATO (Solanum tuberosum L.) CULTIVARS BY PROTEIN AND RAPD MARKERS
Author(s) -
Antar El-Banna,
Ismael A. Khatab
Publication year - 2013
Publication title -
egyptian journal of genetics and cytology
Language(s) - English
Resource type - Journals
ISSN - 0046-161X
DOI - 10.21608/ejgc.2013.10459
Subject(s) - rapd , solanum tuberosum , biology , cultivar , genetic diversity , botany , genetic distance , genetic variation , horticulture , genetics , gene , population , demography , sociology
Genetic diversity among nine potato cultivars was investigated using protein and RAPD markers. The electrophoretic pattern of protein analysis produced 28 bands distributed in all cultivars with molecular weights ranging from 21.30 KDa to 112.6 KDa. Some cultivars possessed some bands which were absent in other cultivars. Furthermore, RAPD analysis revealed a high level of polymorphism among the studied genotypes. A total number of 308 amplified bands were generated across the studied genotypes with average of 12.83 bands/primer. SDS-protein marker produced a dendrogram almost similar to that obtained by the RAPD analysis. It could be concluded that, both of SDS-Protein and RAPD markers are equally important for genetic analysis and indicate a considerable amount of genetic diversity between the different studied varieties of potato.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom