Biochemical changes in proteins and DNA in Ctenopharyngodon idella in response to treatment with the biopesticide "Triology®".

This work was planned to study the biochemical effects of neem biopesticide "Triology ® " on plasma albumin, plasma total protein, heat shock protein70, and DNA content in liver of the fresh water fish, "Ctenopharyngodon idella" Fish were divided into 3 groups. The first group was exposed to 1/10 LC50 (11.2 mg/L) of the pesticide "neem". Fish were sampled at intervals of 5, 10 and 15 days post exposure. Some fish were left for recovery, and sampled at the intervals of 5 and 10 days. The second group was exposed to 1/2 LC50 (56 mg/L) of the pesticide and were sampled after successive intervals of 2, 4 and 6 days of exposure. Some fish were left for recovery, and sampled after the intervals of 3 and 6 days. The third group served as control. The biochemical results revealed a great decline in plasma albumin, plasma total protein, and DNA content in the liver, as well as an enhancement of hsp70 gene which translated to increased amounts of heat shock protein (hsp70) in the neem-treated groups. The recovery studies showed a partial to complete recovery in the investigated parameters, but not in the same manner.