Evaluation of Lactate Dehydrogenase Activity During Orthodontic Tooth Movement

Objective: This study was to evaluate the activity of Lactate Dehydrogenase enzyme in different tooth malpositions in gingival crevicular fluid (GCF). Patients, materials and methods: A sample of 18 patients with an age range from 13 to 19years, requiring orthodontic treatment. The study groups were designed into two groups. Group I: well aligned teeth (control group). Group II: malposed teeth. Group II was subsequently divided into 3 sub-groups; Rotation, Spacing and Vertical malposition. Samples were collected for assessment of lactate dehydrogenase level in GCF at baseline, one week, two and four weeks after treatment. Lactate dehydrogenase was measured in GCF using Enzyme Linked Immunosorbent Assay (ELISA) technique. Results: In both groups (group I and group II subgroups) the gingival crevicular fluid lactate dehydrogenase enzyme had a steady increase during orthodontic tooth movement with a statistically significant increase at one week and two weeks compared with the base line (P≤0.05). No statistical significant difference was found between each two subgroups of group II (P≤ 0.05). Conclusions: The level of lactate dehydrogenase enzyme in gingival crevicular fluid could be used as a biomarker for periodontal metabolism related to orthodontic tooth movement. The level of lactate dehydrogenase enzyme (LDH) did not show significant difference among different tooth malpositions during correction by orthodontic treatment.