Production and Characterization of Xylanase from Streptomyces sp. which is Isolated from Soil.

In this work, Streptomyces sp., was isolated from soil, identified and optimized. Optimum conditions of Streptomyces sp. were determined and it is observed that maximum reproduction is achieved at 35 C, pH 7.0 and 52 hours. It was also revealed that the best conditions for producing xylanase was 35 C, pH 7.0 and 72 hours and the best enzyme activity was achieved at 40 °C and pH 7.0. The effect of metals on enzyme activity was investigated, and it was observed that enzyme activity was strongly inhibited by Zn , Hg 2+ and Fe . It was found that Triton X-100, one of the detergents was used, boosts activity, while other one, SDS, inhibits strongly and it is revealed that, enzyme activity remains stable for 1 hour at 35C, 40 C and 45C and also it is preserved 100% after 1 hour at pH 6.0 and pH 7.0.