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Determination Of Prokaryotic Diversity Of Salda Lake By Next Generation Sequencing Method
Author(s) -
Nilgün Poyraz,
Mehmet Mutlu
Publication year - 2017
Publication title -
journal of the institute of science and technology
Language(s) - English
Resource type - Journals
ISSN - 2146-0574
DOI - 10.21597/jist.2017.167
Subject(s) - diversity (politics) , computational biology , dna sequencing , biology , evolutionary biology , ecology , genetics , gene , sociology , anthropology
In our study, water samples were taken from Salda Lake and the pH and salinity data for the sample were determined. After that, samples were filtered and DNA extraction was performed. The obtained DNA was sequenced by the Illumina MiSeq sequencing system and data was analyzed by the QIIME program. When the data obtained after the analysis were evaluated, it was determined that the most dominant bacterial phyla are Proteobacteria and Actinobacteria. At class level, Gammaproteobacteria, Acidimicrobia and Actinobacteria were dominant. In order level Enterobacteriales, Acidimicrobiales, Actinobacteriales were abundant. When the Archaea domain was evaluated it was determined that the Euryarchaeota phylum was dominant. Halobacteria members were abundant at the class level. In addition Halobacteria class, Halobacteriales order, Halobacteriaceae family includes Haloarcula, Halobacterium, Halonotius, Halorhabdus, Halorubrum and Haloplanus genus members.

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