Investigation the Efficacy of Nano Silver Sulfadiazine and Benzalkonium Chloride Disinfectants on Planktonic Bacteria and Biofilm Layer

Rapid developing nanotechnology has provided creation of new products in nano size finding usage in science and technology applications. Nanoparticles provide benefits on different scientific fields as well as using them in production of industrial products. Especially, production of disinfectants using antimicrobial properties of nanoparticles increases against microorganisms that are industrially and clinically important. Silver sulfadiazine and the benzalkonium which are examples of the quaternary ammonium compounds, has been used for a long time as a disinfectant, have been known that they show bacteriostatic and bacteriocidal effect on bacteria, some viruses, fungus and protozoa according to the concentration. Some bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Eschericha coli and Klebsiella pneumoniae are clinically and industrially harmful. In addition, these bacteria can cause economic problems with their biofilm structure. Indefensible planktonic cells are affected by this circumstance very easily. The protected biofilm bacteria continue to resist against the disinfectant. The aim of the recent study is to examine minimum bactericidal concentration (MBC) values of nano silver sulfadiazine and nano benzalkonium chloride against planktonic and biofilm forms of these bacteria to determine time and concentration. The minimal effective dose in biofilms were determined as 512 μl ml for nano silver sulfadiazin and 128 μl ml for nano benzalkonium chloride, respectively. Results of this study were evaluated by the producer to determine the final concentration of nano silver sulfadiazin and nano benzalkonium chloride.