The Role of Curcumin-Loaded Solid Lipid Nanoparticles Labeled with Tc-99m in the Liver-Spleen Scintigraphy

OZET: Son zamanlarda, hastaliklarin tani ve tavisini kolaylastirmak amaciyla, nanoteknoloji ve molekuler goruntuleme alanlari, cok fonksiyonlu nanopartikullerin olusturulmasi ile birlestirilmistir. Nanopartikullerden olusturulan yeni nesil goruntuleme ajanlarinin sahip oldugu cok yonlu ve essiz ozelliklerinden faydalanan alanlardan biriside nukleer tip alanidir. Yapilan bu calismada; kurkumin yuklu kati lipit nanopartikullerin (K-KLN) karaciger ve dalak sintigrafisinde kullanilabilirliligi arastirildi. Oncelikle, kurkumin yuklu kati lipit nanopartikuller (K-KLN) mikroemulsiyon ve dusuk- sicaklikda katilastirma yontemiyle hazirlandi. Lazer kirinimi (LD) analizi ile partikul boyutu belirlendi. Termogravimetrik analizi (TGA) ile K-KLN'lerin sicakliga karsi stabil oldugu gozlemlendi. Daha sonra K-KLN'ler Teknesyum-99m ( 99m Tc) ile radyoaktif olarak isaretlendi. Ince katman kromatografi yontemi ile radyoaktif isaretleme etkinligi %95'den daha fazla olarak bulundu. 99m Tc-K-KLN'ler intravenoz (i.v.) olarak tavsana enjekte edildi ve gama kamera ile dinamik goruntuler kaydedildi. Elde edilen dinamik goruntulerde karaciger ve dalak belirgin bir sekilde goruntulendi. Sintigrafik bulgulara ek olarak yapilan ex vivo biyodagilim calismalari ve floresan mikroskop calismalarinda karaciger ve dalakta 99m Tc-K-KLN'lerin varligi gozlemlendi. Anahtar Kelimeler: Biyodagilim, kati lipit nanopartikul, kurkumin, sintigrafi, Tc-99m ABSTRACT: Recently, nanotechnology and molecular imaging fields are integrated to facilitate the diagnosis and the treatment of diseases with create multi-functional nanoparticles. Nuclear medicine is one of the fields that benefit from the multi-faceted and unique characteristics of new generation imaging agents that are made of nanoparticles. This study investigated the feasibility of using curcumin-loaded SLNs in liver and spleen scintigraphy. Initially, the curcumin-loaded solid lipid nanoparticles (C-SLN) were prepared using microemulsion and solidification at low temperatures. The size of the C-SLN particle is measured by Laser diffraction (LD) method. Moreover, by applying thermogravimetric analysis (TGA) it is observed that C-SLN particles are stable with temperature variation. Then, they are radiolabeled with Technetium-99m ( 99m Tc). Using the thin-layer chromatography method, the radiolabeling efficiency was found to be higher than 95%. 99m Tc-C-SLNs were injected to the rabbit intravenously (i.v.) and using a gamma camera, dynamic images were recorded. Liver and spleen were clearly displayed in these dynamic images. In addition to scintigraphic findings, the presence of 99m Tc-C-SLNs in the liver and the spleen was observed in the ex vivo biodistribution studies and the analyses performed under the fluorescence microscope.