Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers | Zendy

Ming-Kung Yeh | Zendy; Chieh-Shen Hu | Zendy; ChiaoHsi Chiang | Zendy; PoDa Hong | Zendy

Open Access

Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers

Author(s) -

Ming-Kung Yeh,

Chieh-Shen Hu,

ChiaoHsi Chiang,

PoDa Hong

Publication year - 2012

Publication title -

international journal of nanomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.245

H-Index - 128

eISSN - 1178-2013

pISSN - 1176-9114

DOI - 10.2147/ijn.s34770

Subject(s) - nanoparticle , endocytosis , biophysics , chondroitin sulfate , confocal microscopy , zeta potential , bovine serum albumin , cytotoxicity , caco 2 , chitosan , materials science , chemistry , nanotechnology , biochemistry , glycosaminoglycan , cell , in vitro , biology , microbiology and biotechnology

Chondroitin sulfate-chitosan (ChS-CS) nanoparticles and positively and negatively charged fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)-loaded ChS-CS nanoparticles were prepared and characterized. The properties of ChS-CS nanoparticles, including cellular uptake, cytotoxicity, and transepithelial transport, as well as findings on field emission-scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy were evaluated in human epithelial colorectal adenocarcinoma (Caco-2) fibroblasts. ChS-CS nanoparticles with a mean particle size of 250 nm and zeta potentials ranging from -30 to +18 mV were prepared using an ionic gelation method.

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