The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells | Zendy

Daniel Horák | Zendy; Michal Babič | Zendy; Pavla Jendelová | Zendy; Vı́t Herynek | Zendy; Vladimír Proks | Zendy; Vanecek | Zendy; Eva Syková | Zendy

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The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

Author(s) -

Daniel Horák,

Michal Babič,

Pavla Jendelová,

Vı́t Herynek,

Vladimír Proks,

Vanecek,

Eva Syková

Publication year - 2012

Publication title -

international journal of nanomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.245

H-Index - 128

eISSN - 1178-2013

pISSN - 1176-9114

DOI - 10.2147/ijn.s28658

Subject(s) - nanoparticle , coprecipitation , mesenchymal stem cell , maghemite , materials science , nuclear chemistry , chemistry , stem cell , biophysics , nanotechnology , medicine , pathology , microbiology and biotechnology , inorganic chemistry , biology

Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe(2)O(3)) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe(2)O(3) surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe(2)O(3) and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe(2)O(3) nanoparticles with DPA-HA/γ-Fe(2)O(3) = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.

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