Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells | Zendy

Alexandru Biris | Zendy; Karmakar | Zendy; Braton | Zendy; Dervishi | Zendy; Ghosh | Zendy; Mahmood Mahmood | Zendy; Saeed | Zendy; Thikra Mustafa | Zendy; Daniel A. Casciano | Zendy; Radominska-Pandya | Zendy

Open Access

Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells

Author(s) -

Alexandru Biris,

Karmakar,

Braton,

Dervishi,

Ghosh,

Mahmood Mahmood,

Saeed,

Thikra Mustafa,

Daniel A. Casciano,

Radominska-Pandya

Publication year - 2011

Publication title -

international journal of nanomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.245

H-Index - 128

eISSN - 1178-2013

pISSN - 1176-9114

DOI - 10.2147/ijn.s17684

Subject(s) - acridine orange , ethidium bromide , apoptosis , in vitro , oncogene , microbiology and biotechnology , carbon nanotube , gene delivery , ethylenediamine , cancer cell , materials science , mcf 7 , gene , biophysics , cancer research , chemistry , nanotechnology , biology , transfection , dna , cell cycle , cancer , biochemistry , human breast , genetics , inorganic chemistry

A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(-1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.

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