In vitro determination of the antibiotic susceptibility of biofilm-forming Pseudomonas aeruginosa and Staphylococcus aureus: possible role of proteolytic activity and membrane lipopolysaccharide | Zendy

Majed M. Masadeh | Zendy; Mhaidat | Zendy; Karem H. Alzoubi | Zendy; Emad Hussein | Zendy; Al-Trad | Zendy

Open Access

In vitro determination of the antibiotic susceptibility of biofilm-forming Pseudomonas aeruginosa and Staphylococcus aureus: possible role of proteolytic activity and membrane lipopolysaccharide

Author(s) -

Majed M. Masadeh,

Mhaidat,

Karem H. Alzoubi,

Emad Hussein,

Al-Trad

Publication year - 2013

Publication title -

infection and drug resistance

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.033

H-Index - 39

ISSN - 1178-6973

DOI - 10.2147/idr.s41501

Subject(s) - biofilm , pseudomonas aeruginosa , microbiology and biotechnology , staphylococcus aureus , antibiotics , protease , bacteria , lipopolysaccharide , biology , chemistry , biochemistry , enzyme , immunology , genetics

We carried out a comprehensive overview of inhibitory effects of selected antibiotics on planktonic and biofilm cells of Staphylococcus aureus (ATCC 29213) and Pseudomonas aeruginosa (ATCC 27853) strains. The possible involvement of protease activity and the lipopolysaccharide (LPS) profile of P. aeruginosa were also analyzed. Biofilm cells of both strains were more resistant to antibiotics than their planktonic counterparts. Protease activity was increased in both strains in the biofilm forms. Challenge with sublethal doses of antibiotics also increased proteolytic activity of biofilm cells. Additionally, the LPS profile of P. aeruginosa showed pattern alterations of the biofilm that can contribute to biofilm resistance and survival. These observations provide evidence for the involvement of bacterial proteolytic activity and LPS profile in the resistance of biofilm bacteria to antibiotics compared to their planktonic counterparts.

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