A novel plasmid carrying carbapenem-resistant gene blaKPC-2 in Pseudomonas aeruginosa | Zendy

Yanyan Hu | Zendy; Qi Wang | Zendy; Qiaoling Sun | Zendy; Gong-Xiang Chen | Zendy; Rong Zhang | Zendy

Open Access

A novel plasmid carrying carbapenem-resistant gene blaKPC-2 in Pseudomonas aeruginosa

Author(s) -

Yanyan Hu,

Qi Wang,

Qiaoling Sun,

Gong-Xiang Chen,

Rong Zhang

Publication year - 2019

Publication title -

infection and drug resistance

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.033

H-Index - 39

ISSN - 1178-6973

DOI - 10.2147/idr.s196390

Subject(s) - pseudomonas aeruginosa , plasmid , microbiology and biotechnology , gene , biology , bacteria , genetics

A carbapenem-resistant strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of gene (ΔIS-Tn-IS -IS-IS).

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