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A carbapenem-resistant  strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the   gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene   pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of   gene (ΔIS-Tn-IS -IS-IS).

infection and drug resistance

&lt;p&gt;A novel plasmid carrying carbapenem-resistant gene &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;KPC-2&lt;/sub&gt; in &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt;&lt;/p&gt;