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begs some questions. Although the limitations of the lifting agent have been correctly identified by the authors, it would be useful to know the timing and details of the procedures used for preparation of blood, plasma, and serum. The quality of the agent used suggests that it behaves, in terms of viscosity and transparency, like a hematoma at the time of endoscopic resection. Thus, endoscopic visibility for detecting the mucosal layers may be affected by the lifting agent, especially when the amount of fluid used exceeds 1 4mL. Rightly, the authors emphasize that methylene or toluidine blue colorants can be used during submucosal resection without impairing visibility. However, they also have the advantage of highlighting the different wall layers according to different rates of absorption, and they are used in a strong concentration.As demonstrated by other studies,

Is long-lasting mucosal elevation the only valid parameter when evaluating a lifting agent?