Extraction of antioxidative principles of Achillea biserrata M. Bieb. and chromatographic analyses

In this study, hexane, chloroform and water extracts were prepared by making consecutive extraction of Achillea biserrata from the family Asteraceae. Volatile oil of the same types was obtained by water distillation that was continued 4 hours in the Clevenger fixture and dried by adding anhydrous sodium sulfate. After GS/MS analysis, volatile oil of the same types was kept at 4 oC in the refrigerator and dark until the work activity was performed. Volatile oil yield that was obtained from mixture of all dried flower and plant leaves were determined for Achillea biserrata 0.4%(v/w) The antioxidant activities of extracts, total phenol, flavonoid, proanthocyanidin and anthocyanin amounts were determined. Two different tests were performed for measurements of antioxidant activities.1 Especially, for the purpose of meeting the general properties of good additives’ free radical cleaning activity was done by DPPH method and the inhibition of oxidation activity was done by with β-carotene method. The 50% inhibition (IC50) values of the water, methanol:water and chloroform extracts were 19.6, 37.9 and 114 μg /mL, respectively in DPPH test. Besides, the nonpolar extracts were active in β-carotene/linoleic acid test system. Total phenolics and total flavonoid contents were highest in the water extract possessing 3.39% and 1.12%, respectively. Methanolic extract showed the presence of antioxidant phenolic compounds such as protocatecuic acid (177.83 g/g) and chlorogenic acid (164.78 g/g) in HPLC analsis. Automated extraction system obtained better separation of the active principles from plant tissues than conventional extraction procedures.