A proper placental sampling for syncytin-1 analysis | Zendy

Petra Priščáková | Zendy; Miroslav Korbeľ | Zendy; Z Nižňanská | Zendy; Katarína Letkovská | Zendy; K. Susienkova | Zendy; Vanda Repiská | Zendy; Daniel Böhmer | Zendy; Helena Gbelcová | Zendy

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A proper placental sampling for syncytin-1 analysis

Author(s) -

Petra Priščáková,

Miroslav Korbeľ,

Z Nižňanská,

Katarína Letkovská,

K. Susienkova,

Vanda Repiská,

Daniel Böhmer,

Helena Gbelcová

Publication year - 2020

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2020-0121

Subject(s) - placenta , andrology , sampling (signal processing) , umbilical cord , biopsy , biology , obstetrics , placenta diseases , syncytiotrophoblast , pregnancy , medicine , fetus , pathology , immunology , genetics , computer science , filter (signal processing) , computer vision

Syncytin-1 (gene ERVW-1) has been proposed as a marker of pre-eclampsia and malfunctions in placental development. Placenta is heterogeneous tissue, hence the method of biopsy can significantly affect the outcome of analyses. A total of 44 placentae were analyzed by taking 3–30 samples from each. Relative levels of ERVW-1 expression in the placental biopsies were characterized by RT-qPCR. Evaluation of ten biopsies from one placenta individually (not pooling them) is recommended due to the high variability of expression. No significant correlation was found between biopsy localization and level of ERVW-1 expression; therefore, random sampling is recommended. A long cut from the umbilical cord to the edge of the placenta is a convenient approach to placental sampling.

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