Vacuum Microcasting of 2-Methacryloyloxyethyl Phosphorylcholine Polymer for Stable Cell Patterning | Zendy

Nobuyuki Tanaka | Zendy; Ryoji Sekine | Zendy; Shunichi Funano | Zendy; Asako Sato | Zendy; Núria Taberner | Zendy; Miki Ebisuya | Zendy; Yo Tanaka | Zendy

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Vacuum Microcasting of 2-Methacryloyloxyethyl Phosphorylcholine Polymer for Stable Cell Patterning

Author(s) -

Nobuyuki Tanaka,

Ryoji Sekine,

Shunichi Funano,

Asako Sato,

Núria Taberner,

Miki Ebisuya,

Yo Tanaka

Publication year - 2020

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2020-0052

Subject(s) - polydimethylsiloxane , phosphorylcholine , materials science , polymer , adhesion , fabrication , mold , cell adhesion , microstructure , chemical engineering , nanotechnology , composite material , chemistry , medicine , biochemistry , alternative medicine , pathology , engineering

This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.

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