Open AccessParallel sample processing using dispersive INtip micro-purification on programmable multichannel pipettes
Author(s) -
Patrick A. Kates,
John J. Tomashek,
David A. Miles,
L. Andrew Lee
Publication year - 2020
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2019-0140
Subject(s) - pipette , automation , throughput , computer science , microfluidics , scalability , workflow , process engineering , chromatography , nanotechnology , materials science , chemistry , engineering , mechanical engineering , telecommunications , database , wireless
Automation gives researchers the ability to process and screen orders of magnitude higher numbers of samples than manual experimentation. Current biomacromolecule separation methodologies suffer from necessary manual intervention, making their translation to high-throughput automation difficult. Herein, we present the first characterization of biomacromolecule affinity purification via dispersive solid-phase extraction in a pipette tip (INtip). We use commercially available resin and compare efficiency with batch and spin column methodologies. Moreover, we measure the kinetics of binding and evaluate resin binding capacities. INtip technology is effective on, and scalable for, an automated platform (INTEGRA ASSIST). The results suggest that high-throughput biomolecular workflows will benefit from the integration of INtip separations.