Open AccessNanoliter-scale next-generation sequencing library-mediated high-throughput 16S rRNA microbial community profiling
Author(s) -
Hui Zhang,
Xiangdan Yu,
Zhe Zhang,
Zhenhua Liu,
Cong Tang,
Kun Zhao,
Shiyan Liu,
Yong Zhen Sun,
Xiang Li,
Zijing Xu,
Hong Zhang,
Keqing Shi,
Yan Fu
Publication year - 2020
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2019-0102
Subject(s) - amplicon sequencing , amplicon , biology , computational biology , pooling , dna sequencing , profiling (computer programming) , metagenomics , workflow , ribosomal rna , deep sequencing , illumina dye sequencing , 16s ribosomal rna , genetics , polymerase chain reaction , gene , computer science , database , genome , artificial intelligence , operating system
An ultra-high-throughput workflow for next-generation sequencing library construction at nanoliter scale for amplicon sequencing, termed Smartchip Nanowell Platform for Target Enrichment, was established using a nanodispenser system and a nanoliter-scale PCR chip. To demonstrate its cost and time advantages over conventional methods for library construction, quality control and pooling for large-scale samples, target amplicon sequencing of the 16S ribosomal RNA gene V3-V4 region widely used for microbial community profiling was chosen for comparison. The finding of no significant difference in microbial community profiling between the two methods strongly supports the conclusion that Smartchip Nanowell Platform for Target Enrichment is a cost-effective method for next-generation sequencing library construction for large-scale samples to conduct amplicon sequencing-based applications.
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