A rapid cell-based assay for determining poloxamer quality in CHO suspension cell culture | Zendy

Athmaram Thimmasandra Narayanappa | Zendy; Sam Mwilu | Zendy; Stacy Holdread | Zendy; Kimesha Hammett | Zendy; George Bu | Zendy; Elizabeth Chen Dodson | Zendy; James W. Brooks | Zendy

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A rapid cell-based assay for determining poloxamer quality in CHO suspension cell culture

Author(s) -

Athmaram Thimmasandra Narayanappa,

Sam Mwilu,

Stacy Holdread,

Kimesha Hammett,

George Bu,

Elizabeth Chen Dodson,

James W. Brooks

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2019-0070

Subject(s) - poloxamer , chromatography , cell culture , cell , cell adhesion , membrane , chemistry , viability assay , bioprocess , cell growth , poloxamer 407 , polymer , chemical engineering , biochemistry , biology , organic chemistry , copolymer , engineering , genetics

Poloxamers are water-soluble polymers that are widely used in cell culture bioprocessing to protect cells against shearing forces. Use of poor-quality poloxamers may lead to a drastic reduction in cell growth, viabilities and productivities in cell culture-based manufacturing. In order to evaluate poloxamer quality and promote more consistent performance, a rapid cell membrane adhesion to hydrocarbon assay was developed based on the adhesive properties of cell membranes to selective hydrocarbons. The assay can identify a poor-performing poloxamer characterized by significant drop in viable cell density and percent viability. The assay was verified across multiple good and bad poloxamer lots, and the results were in agreement with established cell growth and high-performance liquid chromatography assays.

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