Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site | Zendy

Jens Staal | Zendy; Kübra Alci | Zendy; Wouter De Schamphelaire | Zendy; Martine Vanhoucke | Zendy; Rudi Beyaert | Zendy

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Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site

Author(s) -

Jens Staal,

Kübra Alci,

Wouter De Schamphelaire,

Martine Vanhoucke,

Rudi Beyaert

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2019-0014

Subject(s) - cloning (programming) , plasmid , cloning vector , multiple cloning site , restriction enzyme , genetics , restriction site , biology , vector (molecular biology) , computational biology , molecular cloning , mutagenesis , synthetic biology , recombinant dna , gene , mutation , computer science , peptide sequence , programming language

Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185-bp fully functional high-copy cloning plasmid with an extended multiple cloning site. We believe that this is the smallest high-copy cloning vector ever described.

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