Development and Optimization of a High Titer Recombinant Lentivirus System | Zendy

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Development and Optimization of a High Titer Recombinant Lentivirus System

Author(s) -

Mirus Bio LLC

Publication year - 2018

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-2003

Subject(s) - lentivirus , recombinant dna , titer , transfection , virology , biology , vector (molecular biology) , microbiology and biotechnology , cell culture , virus , genetics , gene , viral disease

To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time. Adherent or suspension adapted 293-derived cells can be used as production hosts. These results illustrate the importance of optimizing transfection processes for high titer recombinant lentivirus production.

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