The Effectiveness of Temperature Control of Thermocyclers in PCR Optimization | Zendy

Elisa A Santos | Zendy; Roberto Macoto Ichinose | Zendy; Liz Maria de Almeida | Zendy

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The Effectiveness of Temperature Control of Thermocyclers in PCR Optimization

Author(s) -

Elisa A Santos,

Roberto Macoto Ichinose,

Liz Maria de Almeida

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0177

Subject(s) - myeloid leukemia , control sample , sample (material) , polymerase chain reaction , cancer , biology , oncology , medicine , immunology , genetics , gene , chromatography , chemistry , food science

This study evaluated the effectiveness of thermocycler temperature control, considering the influence of other determinant factors for the optimization of PCR. The reduction in the number of repeated PCR tests, applied in the diagnosis and prognosis of chronic myeloid leukemia at the National Cancer Institute in Brazil, was used as a measure of effectiveness. This indicator was evaluated using samples obtained before and after the temperature control in the wells of the thermocyclers. There was a reduction of 18.9% in the number of repeated exams in the second sample. A structured interview with laboratory staff indicated that there was no change in the other determinant factors.

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