A method for rapid analysis of the root hydrotropic response in Arabidopsis thaliana | Zendy

L Noriega-Calixto L | Zendy; María E. Campos | Zendy; Gladys I. Cassab | Zendy

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A method for rapid analysis of the root hydrotropic response in Arabidopsis thaliana

Author(s) -

L Noriega-Calixto L,

María E. Campos,

Gladys I. Cassab

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0173

Subject(s) - petri dish , arabidopsis thaliana , arabidopsis , mutant , curvature , moisture , chemistry , biophysics , biology , biological system , biochemistry , mathematics , genetics , gene , geometry , organic chemistry

The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type Arabidopsis roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of K 2 CO 3 with a density of 1.48 g·ml -1 to generate a rapid moisture gradient inside a square Petri dish without decreasing root growth. In this, the root growth rate observed in hydrostimulated wild-type and miz1 mutant, utilized as a negative control, increases sixfold compared with those roots examined using the former method.

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