An inexpensive air stream temperature controller and its use to facilitate temperature-controlled behavior in Drosophila | Zendy

Ryan Sangston | Zendy; Jay Hirsh | Zendy

Open Access

An inexpensive air stream temperature controller and its use to facilitate temperature-controlled behavior in Drosophila

Author(s) -

Ryan Sangston,

Jay Hirsh

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0152

Subject(s) - drosophila melanogaster , drosophila (subgenus) , biology , biological neural network , dopaminergic , neuroscience , microbiology and biotechnology , computational biology , biochemistry , gene , dopamine

Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.

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