Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions | Zendy

Pranvera Hiseni | Zendy; Robert C. Wilson | Zendy; Ola Storr⊘ | Zendy; Roar Johnsen | Zendy; Torbj⊘rn Øien | Zendy; Knut Rudi | Zendy

Open Access

Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions

Author(s) -

Pranvera Hiseni,

Robert C. Wilson,

Ola Storr⊘,

Roar Johnsen,

Torbj⊘rn Øien,

Knut Rudi

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0134

Subject(s) - multiplex , bacteroides , detection limit , bacteroidaceae , molecular diagnostics , chromatography , multiplex polymerase chain reaction , biology , tube (container) , polymerase chain reaction , microbiology and biotechnology , materials science , chemistry , bacteria , bioinformatics , genetics , gene , composite material

We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction. We evaluated LAD both on pure cultures, and on infant gut microbiota for a 15-plex reaction. LAD showed more than 80% accuracy of classification and a detection limit lower than 2% of the Illumina reads per sample. The results on the clinical dataset showed that there was a rapid decrease of staphylococci from 10-day- to 4-month-old children, a peak of bifidobacteria at 4 months, and a peak of Bacteroides in 2-year-old children, which is in accordance with findings described in the literature. Being able to detect up to 50 biomarkers, LAD is a suitable method for assays where high throughput is essential.

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