Capture and storage of plant genomic DNA on a readily available cellulose matrix | Zendy

Megan M. Thompson | Zendy; Estelle M. Hrabak | Zendy

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Capture and storage of plant genomic DNA on a readily available cellulose matrix

Author(s) -

Megan M. Thompson,

Estelle M. Hrabak

Publication year - 2018

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0109

Subject(s) - nucleic acid , cellulose , dna , matrix (chemical analysis) , reagent , genomic dna , chromatography , chemistry , biochemistry , organic chemistry

Whatman FTA ® Cards are a fast and efficient method for capturing and storing nucleic acids but can be cost-prohibitive for large numbers of samples. We developed a method that substitutes a readily-available cellulose matrix and homemade washing buffer for commercial FTA ® Cards and FTA ® Purification Reagent. This method is suitable for long-term storage of DNA from many plant species prior to PCR.

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