Single nucleotide recognition using a probes-on-carrier DNA chip | Zendy

Saifullah Saifullah | Zendy; Satoshi Fuke | Zendy; Hiroshi Nagasawa | Zendy; Toshifumi Tsukahara | Zendy

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Single nucleotide recognition using a probes-on-carrier DNA chip

Author(s) -

Saifullah Saifullah,

Satoshi Fuke,

Hiroshi Nagasawa,

Toshifumi Tsukahara

Publication year - 2019

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0088

Subject(s) - nucleotide , dna , chip , microbiology and biotechnology , genetics , biology , computational biology , computer science , gene , telecommunications

Following the sequencing of the human genome, SNP analysis of individual patients has become essential for achieving the best drug response and ensuring optimal care. In this study, we developed a cost-effective probes-on-carrier DNA chip for the detection of SNPs. Our chips harbored three different probes against the TP53 gene, and were capable of detecting wild-type TP53 and SNPs such as rs121912651 and rs11540652. Four cell lines were used to validate the specificity of probe hybridization. Strong fluorescence intensity was observed in hybridized spots based on hybridization for perfect base pairing between complementary strands, whereas significantly lower fluorescence (p < 0.05) was observed in nonhybridized spots. These hybridization results indicated that the probes-on-carrier chip is suitable for SNP genotyping.

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