A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings | Zendy

Shia Vang | Zendy; Kati Seitz | Zendy; Patrick J. Krysan | Zendy

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A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings

Author(s) -

Shia Vang,

Kati Seitz,

Patrick J. Krysan

Publication year - 2018

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0044

Subject(s) - arabidopsis , live cell imaging , microfluidics , calcium imaging , plant cell , biology , microbiology and biotechnology , botany , biophysics , cell , nanotechnology , chemistry , calcium , materials science , biochemistry , gene , organic chemistry , mutant

One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell ™ can be repurposed to create an imaging chamber suitable for Arabidopsis seedlings, cotyledons and leaves. Liquid in the imaging chamber can be rapidly exchanged to introduce chemical treatments via microfluidic passive pumping. When used in conjunction with fluorescent biosensors, this system can facilitate live-cell imaging studies of signal transduction pathways triggered by different treatments. As a demonstration, we show how the HybriWell can be used to monitor flg22-induced calcium transients using the R-GECO1 calcium indicator in detached Arabidopsis leaves.

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