Absolute bioluminescence imaging at the single-cell level with a light signal at the Attowatt level | Zendy

Toshiteru Enomoto | Zendy; Hidehiro Kubota | Zendy; Kaneo Mori | Zendy; Masahiro Shimogawara | Zendy; Masahiro Yoshita | Zendy; Yoshihiro Ohmiya | Zendy; Hidefumi Akiyama | Zendy

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Absolute bioluminescence imaging at the single-cell level with a light signal at the Attowatt level

Author(s) -

Toshiteru Enomoto,

Hidehiro Kubota,

Kaneo Mori,

Masahiro Shimogawara,

Masahiro Yoshita,

Yoshihiro Ohmiya,

Hidefumi Akiyama

Publication year - 2018

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0043

Subject(s) - bioluminescence , signal (programming language) , bioluminescence imaging , luciferase , light source , optics , physics , biophysics , chemistry , biology , computer science , cell culture , transfection , biochemistry , genetics , programming language

Bioluminescence imaging (BLI) demonstrates cellular events as a light signal at the single-cell level using a highly sensitive, cooled CCD camera. However, BLI signals are relative values and thus, images taken on different days or using different equipment cannot be compared directly. We established a reference LED light source that was characteristic of the total flux and light distribution and calibrated the BLI system as an absolute light signal. This calibrated BLI system revealed that the average light signal of beetle luciferase was at an attowatt level per sec at the single cell level.

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