Long-Term Imaging of Calcium Dynamics Using Genetically Encoded Calcium Indicators and Automatic Tracking of Cultured Cells | Zendy

I. N. Razlivanov | Zendy; Teresa Liew | Zendy; Eira Watts Moore | Zendy; Alaa Al-Kathiri | Zendy; Tayma Bartram | Zendy; Dmitriy Kuvshinov | Zendy; Anton Nikolaev | Zendy

Open Access

Long-Term Imaging of Calcium Dynamics Using Genetically Encoded Calcium Indicators and Automatic Tracking of Cultured Cells

Author(s) -

I. N. Razlivanov,

Teresa Liew,

Eira Watts Moore,

Alaa Al-Kathiri,

Tayma Bartram,

Dmitriy Kuvshinov,

Anton Nikolaev

Publication year - 2018

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2018-0024

Subject(s) - calcium , calcium imaging , calcium signaling , live cell imaging , microbiology and biotechnology , biology , cell , dynamics (music) , computational biology , signal transduction , chemistry , biochemistry , physics , organic chemistry , acoustics

Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics. Here we report a methodological approach of long-term (5–10 h) imaging of calcium dynamics in cultured cells. The approach links calcium imaging using genetically encoded calcium indicators and semi-automatic tracking of individual cells. It can be used in a large variety of situations, ranging from the role of calcium in biological processes to cell heterogeneity and screening of drugs modifying signaling pathways.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research