Confocal Laser Scanning Microscopy | Zendy

Stephen W. Paddock | Zendy

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Confocal Laser Scanning Microscopy

Author(s) -

Stephen W. Paddock

Publication year - 1999

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/99275ov01

Subject(s) - microscope , confocal , confocal laser scanning microscopy , microscopy , laser scanning , confocal microscopy , materials science , laser , laser microscopy , fluorescence microscope , scanning confocal electron microscopy , optics , light sheet fluorescence microscopy , optical sectioning , resolution (logic) , two photon excitation microscopy , fluorescence , biomedical engineering , computer science , artificial intelligence , physics , medicine

Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photostable fluorescent probes. Such advances have made possible novel experimental approaches for multiple label fluorescence, live cell imaging and multidimensional microscopy.

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