Detection of DNA Damage and Identification of UV-Induced Photoproducts Using the CometAssay™ Kit | Zendy

M. Lemay | Zendy; K Wood | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Detection of DNA Damage and Identification of UV-Induced Photoproducts Using the CometAssay™ Kit

Author(s) -

M. Lemay,

K Wood

Publication year - 1999

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/99274pf01

Subject(s) - comet assay , lysis , dna damage , agarose , dna , alkaline lysis , microbiology and biotechnology , ultraviolet irradiation , ultraviolet , microscope slide , gel electrophoresis , chemistry , biology , irradiation , microscope , biochemistry , physics , optics , dna vaccination , nuclear physics , plasmid

We introduce the first commercially available comet assay for the detection and quantification of DNA damage in individual eukaryotic cells. The major difficulty of the comet assay is the preparation of the slides needed to immobilize the samples throughout the lysis and electrophoretic procedures. The CometAssay kit uses a proprietary technology to precoat glass microscope slides to allow direct application of the agarose embedded sample without any additional slide treatment. In this report, we discuss the detection of DNA damage in individual cells exposed to ultraviolet irradiation using the new CometSlides and their cost compared to traditional slides.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research